Journal: Scientific Reports

Article Title: A common theme in extracellular fluids of beetles: extracellular superoxide dismutases crucial for balancing ROS in response to microbial challenge

doi: 10.1038/srep24082

Figure Lengend Snippet: Thirty larvae were injected each with 200 ng of PcSOD3.1 -, PcSOD3.2-, PcSOD1- dsRNA or gfp -dsRNA. Five days after this initial injection, larvae were challenged with microbes. The probability of survival is depicted as Kaplan-Meier curves by applying Gehan-Breslow statistic test. The weight of freshly emerged pupa was determined after RNAi silencing and infection of larvae. ( A ) Survival upon fungal challenge. After emptying of the defence reservoirs, the larvae of each group were submerged at the same time in the same solution of M. anisopliae 1*10 6 conidia/ml. Overall level of significance: p = 0.014 (Gehan-Breslow test); p = 0.04 (Log Rank (Mantel-Cox)). Pairwise multiple comparison procedures for gfp vs. Pc SOD3.1(−): p = 0.04 (Holm-Sidak method). Pupal weight, level of significance: p = 0.023* (two-tailed t-test). ( B ) Survival after injection of 1*10 6 E. coli -cells/200 nl. ( C ) Survival after injection of 1*10 6 M. luteus -cells/200 nl.

Article Snippet: Metarhizium anisopliae (DSM-1490, DSMZ, Braunschweig, Germany) was maintained on potato-dextrose-agar (Sigma, 2% glucose), and mycelia were used for subsequent propagation.

Techniques: Injection, Infection, Comparison, Two Tailed Test

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Composite analysis to screen the core species-associated to IMF deposition. (A) The LEfSe analysis of intestinal bacterial communities. LEfSe analysis was performed by LEfSe software. (B) Venn diagrams of species. Upward arrows (red) showed that the relative abundances of microbial species were significantly increased by HFMT. (C to F) Bar plot of Bacteroides_uniformis , Sphaerochaeta_globosa , Hydrogenoanaerobacterium_saccharovorans , and Pyramidobacter_piscolens relative abundance, respectively. (G) Spearman correlation analysis between relative abundance of core bacterial species with fat deposition traits in HFMT pigs. ∗ , Spearman correlation test P < 0.05. HFMT, high dose of fecal microbiota transplantation.

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Software, Transplantation Assay

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Intestinal microbes administration changes fat deposition in mice. (A) Weights of soleus, gastrocnemius, tibialis anterior (TA), quadriceps, and extensor digitorum longus (EDL) muscles from mice ( n = 13). (B) Intramuscular fat content in Quadriceps ( n = 5). (C) Cd36 , Dgat2 , and Fasn mRNA expression levels in muscle, respectively. Data are expressed as means ± SEM. Data were analyzed using two-tailed unpaired Student t -test and were considered statistically significant at ∗ P < 0.05 between indicated groups. Cd36 = cluster of differentiation 36; Dgat2 = diacylglycerol O-acyltransferase 2; Fasn = fatty acid synthase. Ctrl, control; SCFA, mice were inoculated with short-chain fatty acid; B.uni , mice were inoculated with Bcteroides uniformis ; S.glo , mice were inoculated with Sphaerochaeta globosa ; P.pis , mice were inoculated with Pyramidobacter piscolens ; H.sac , mice were inoculated with Hydrogenoanaerobacterium saccharovorans ; Mix, mice were inoculated with the mixture of B. uniformis , S. globosa , P. piscolens , and H. saccharovorans .

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Muscles, Expressing, Two Tailed Test, Control

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Composite analysis to screen the core species-associated to IMF deposition. (A) The LEfSe analysis of intestinal bacterial communities. LEfSe analysis was performed by LEfSe software. (B) Venn diagrams of species. Upward arrows (red) showed that the relative abundances of microbial species were significantly increased by HFMT. (C to F) Bar plot of Bacteroides_uniformis , Sphaerochaeta_globosa , Hydrogenoanaerobacterium_saccharovorans , and Pyramidobacter_piscolens relative abundance, respectively. (G) Spearman correlation analysis between relative abundance of core bacterial species with fat deposition traits in HFMT pigs. ∗ , Spearman correlation test P < 0.05. HFMT, high dose of fecal microbiota transplantation.

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Software, Transplantation Assay

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Intestinal microbes administration changes fat deposition in mice. (A) Weights of soleus, gastrocnemius, tibialis anterior (TA), quadriceps, and extensor digitorum longus (EDL) muscles from mice ( n = 13). (B) Intramuscular fat content in Quadriceps ( n = 5). (C) Cd36 , Dgat2 , and Fasn mRNA expression levels in muscle, respectively. Data are expressed as means ± SEM. Data were analyzed using two-tailed unpaired Student t -test and were considered statistically significant at ∗ P < 0.05 between indicated groups. Cd36 = cluster of differentiation 36; Dgat2 = diacylglycerol O-acyltransferase 2; Fasn = fatty acid synthase. Ctrl, control; SCFA, mice were inoculated with short-chain fatty acid; B.uni , mice were inoculated with Bcteroides uniformis ; S.glo , mice were inoculated with Sphaerochaeta globosa ; P.pis , mice were inoculated with Pyramidobacter piscolens ; H.sac , mice were inoculated with Hydrogenoanaerobacterium saccharovorans ; Mix, mice were inoculated with the mixture of B. uniformis , S. globosa , P. piscolens , and H. saccharovorans .

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Muscles, Expressing, Two Tailed Test, Control

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Composite analysis to screen the core species-associated to IMF deposition. (A) The LEfSe analysis of intestinal bacterial communities. LEfSe analysis was performed by LEfSe software. (B) Venn diagrams of species. Upward arrows (red) showed that the relative abundances of microbial species were significantly increased by HFMT. (C to F) Bar plot of Bacteroides_uniformis , Sphaerochaeta_globosa , Hydrogenoanaerobacterium_saccharovorans , and Pyramidobacter_piscolens relative abundance, respectively. (G) Spearman correlation analysis between relative abundance of core bacterial species with fat deposition traits in HFMT pigs. ∗ , Spearman correlation test P < 0.05. HFMT, high dose of fecal microbiota transplantation.

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Software, Transplantation Assay

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Intestinal microbes administration changes fat deposition in mice. (A) Weights of soleus, gastrocnemius, tibialis anterior (TA), quadriceps, and extensor digitorum longus (EDL) muscles from mice ( n = 13). (B) Intramuscular fat content in Quadriceps ( n = 5). (C) Cd36 , Dgat2 , and Fasn mRNA expression levels in muscle, respectively. Data are expressed as means ± SEM. Data were analyzed using two-tailed unpaired Student t -test and were considered statistically significant at ∗ P < 0.05 between indicated groups. Cd36 = cluster of differentiation 36; Dgat2 = diacylglycerol O-acyltransferase 2; Fasn = fatty acid synthase. Ctrl, control; SCFA, mice were inoculated with short-chain fatty acid; B.uni , mice were inoculated with Bcteroides uniformis ; S.glo , mice were inoculated with Sphaerochaeta globosa ; P.pis , mice were inoculated with Pyramidobacter piscolens ; H.sac , mice were inoculated with Hydrogenoanaerobacterium saccharovorans ; Mix, mice were inoculated with the mixture of B. uniformis , S. globosa , P. piscolens , and H. saccharovorans .

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Muscles, Expressing, Two Tailed Test, Control

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Composite analysis to screen the core species-associated to IMF deposition. (A) The LEfSe analysis of intestinal bacterial communities. LEfSe analysis was performed by LEfSe software. (B) Venn diagrams of species. Upward arrows (red) showed that the relative abundances of microbial species were significantly increased by HFMT. (C to F) Bar plot of Bacteroides_uniformis , Sphaerochaeta_globosa , Hydrogenoanaerobacterium_saccharovorans , and Pyramidobacter_piscolens relative abundance, respectively. (G) Spearman correlation analysis between relative abundance of core bacterial species with fat deposition traits in HFMT pigs. ∗ , Spearman correlation test P < 0.05. HFMT, high dose of fecal microbiota transplantation.

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Software, Transplantation Assay

Journal: Animal Nutrition

Article Title: Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes

doi: 10.1016/j.aninu.2021.10.010

Figure Lengend Snippet: Intestinal microbes administration changes fat deposition in mice. (A) Weights of soleus, gastrocnemius, tibialis anterior (TA), quadriceps, and extensor digitorum longus (EDL) muscles from mice ( n = 13). (B) Intramuscular fat content in Quadriceps ( n = 5). (C) Cd36 , Dgat2 , and Fasn mRNA expression levels in muscle, respectively. Data are expressed as means ± SEM. Data were analyzed using two-tailed unpaired Student t -test and were considered statistically significant at ∗ P < 0.05 between indicated groups. Cd36 = cluster of differentiation 36; Dgat2 = diacylglycerol O-acyltransferase 2; Fasn = fatty acid synthase. Ctrl, control; SCFA, mice were inoculated with short-chain fatty acid; B.uni , mice were inoculated with Bcteroides uniformis ; S.glo , mice were inoculated with Sphaerochaeta globosa ; P.pis , mice were inoculated with Pyramidobacter piscolens ; H.sac , mice were inoculated with Hydrogenoanaerobacterium saccharovorans ; Mix, mice were inoculated with the mixture of B. uniformis , S. globosa , P. piscolens , and H. saccharovorans .

Article Snippet: Bacteroides uniformis (DSM 6597 T ), Sphaerochaeta globosa (DSM 22777 T ), Hydrogenoanaerobacterium saccharovorans (DSM 24774 T ), and Pyramidobacter piscolens (DSM 21147 T ) were purchased from the DSMZ and cultured according to the manufacturer's instructions.

Techniques: Muscles, Expressing, Two Tailed Test, Control